Difference between revisions of "Part:BBa K3610048:Design"
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===Design Notes=== | ===Design Notes=== | ||
mCherry part is codon optimized for expression in S. cerevisiae. | mCherry part is codon optimized for expression in S. cerevisiae. | ||
| + | Flag tag fused to the mCherry sequence for western blotting | ||
===Source=== | ===Source=== | ||
Revision as of 22:40, 15 October 2020
CORE ectodomain / mCherry C-terminal for S. cerevisiae
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1740
Illegal BamHI site found at 373
Illegal BamHI site found at 1765
Illegal BamHI site found at 2117 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
mCherry part is codon optimized for expression in S. cerevisiae. Flag tag fused to the mCherry sequence for western blotting
Source
BBa_K2809002, BBa_K3610010, BBa_K1150016, BBa_K3610019, BBa_M10019
References
Wang, Lei; Albert, Markus; Einig, Elias; Fürst, Ursula; Krust, Damaris; Felix, Georg (2016): The pattern-recognition receptor CORE of Solanaceae detects bacterial cold-shock protein. In: Nature plants 2, S. 16185. DOI: 10.1038/nplants.2016.185.
Fan, Jin-Yu; Cui, Zong-Qiang; Wei, Hong-Ping; Zhang, Zhi-Ping; Zhou, Ya-Feng; Wang, Yun-Peng; Zhang, Xian-En (2008): Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein–protein interactions in living cells. In: Biochemical and Biophysical Research Communications 367 (1), S. 47–53. DOI: 10.1016/j.bbrc.2007.12.101.