Difference between revisions of "Part:BBa K3570002:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Optimized for S. | + | Optimized for S. cerevisiae |
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | HO HA up/down - S. | + | HO HA up/down - S. cerevisiae genome CrtI - X. dendrorhous genome CrtYB - X. dendrorhous genome Promoter TDH1 - S. cerevisiae genome Terminator cyc1 - S. cerevisiae genome URA3 selective locus - S. cerevisiae genome |
===References=== | ===References=== | ||
Latest revision as of 16:36, 15 October 2020
Provitamin A synthesis from GGPP in S. cerevisiae
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1297
Illegal PstI site found at 2357
Illegal PstI site found at 2627
Illegal PstI site found at 3605 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2012
Illegal NheI site found at 5006
Illegal PstI site found at 2357
Illegal PstI site found at 2627
Illegal PstI site found at 3605 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 738
Illegal BglII site found at 3364
Illegal BglII site found at 5489
Illegal BglII site found at 6585
Illegal BamHI site found at 3157
Illegal XhoI site found at 4943
Illegal XhoI site found at 4984 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1297
Illegal PstI site found at 2357
Illegal PstI site found at 2627
Illegal PstI site found at 3605 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1297
Illegal PstI site found at 2357
Illegal PstI site found at 2627
Illegal PstI site found at 3605
Illegal NgoMIV site found at 2018 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Optimized for S. cerevisiae
Source
HO HA up/down - S. cerevisiae genome CrtI - X. dendrorhous genome CrtYB - X. dendrorhous genome Promoter TDH1 - S. cerevisiae genome Terminator cyc1 - S. cerevisiae genome URA3 selective locus - S. cerevisiae genome