Difference between revisions of "Part:BBa K3570017"
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YPRCtau3 downstream homology arm part shall be used together with YPRCtau3 upstream homology arm part ([https://parts.igem.org/Part:BBa_K3570016 BBa_K3570016]) to target a functional yeast integration locus. When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do a homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI. </p> | YPRCtau3 downstream homology arm part shall be used together with YPRCtau3 upstream homology arm part ([https://parts.igem.org/Part:BBa_K3570016 BBa_K3570016]) to target a functional yeast integration locus. When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do a homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI. </p> | ||
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| + | This sequence was identified from a personal communication with Dr. Jean-Marie Francois. </p> | ||
<h2>Experiments</h2> | <h2>Experiments</h2> | ||
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality. | Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality. | ||
Latest revision as of 14:42, 15 October 2020
YPRCtau3 downstream homologous sequence
Usage
YPRCtau3 downstream homology arm part shall be used together with YPRCtau3 upstream homology arm part (BBa_K3570016) to target a functional yeast integration locus. When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.
This sequence was identified from a personal communication with Dr. Jean-Marie Francois.
Experiments
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
References
S. cerevisiae genome, chromosome XVI, Ty4 LTR region. GenBank: CP046096.1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 625
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]