Difference between revisions of "Part:BBa K3570016"
 
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<p style="text-indent: 40px">
 
YPRCtau3 upstream homology arm part shall be used together with YPRCtau3 downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570017 BBa_K3570017]) to target a functional yeast integration locus. When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.</p>
 
YPRCtau3 upstream homology arm part shall be used together with YPRCtau3 downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570017 BBa_K3570017]) to target a functional yeast integration locus. When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.</p>
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This sequence was identified from a personal communication with Dr. Jean-Marie Francois. </p>
 
<h2>Experiments</h2>
 
<h2>Experiments</h2>
 
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
 
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

Latest revision as of 14:42, 15 October 2020


YPRCtau3 upstream homologous sequence

Usage

YPRCtau3 upstream homology arm part shall be used together with YPRCtau3 downstream homology arm part (BBa_K3570017) to target a functional yeast integration locus. When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.

This sequence was identified from a personal communication with Dr. Jean-Marie Francois.

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References

S. cerevisiae genome, chromosome XVI, LTR region. GenBank: CP046096.1 Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 137
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 137
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 137
  • 1000
    COMPATIBLE WITH RFC[1000]