Difference between revisions of "Part:BBa K3570012"
| Line 9: | Line 9: | ||
<p style="text-indent: 40px"> | <p style="text-indent: 40px"> | ||
| − | The sequence of the geraniol synthase from <i>Catharanthus roseus</i> (CrGES) has been taken from Genbank (JN882024.1) then truncated to the 43 amino acid in order to increase its activity (Jiang <i>et al</i>., 2017).</p> | + | The sequence of the geraniol synthase from <i>Catharanthus roseus</i> (CrGES) has been taken from Genbank (JN882024.1), adapted for the usage in <i>S. cerevisiae</i> and then truncated to the 43 amino acid in order to increase its activity (Jiang <i>et al</i>., 2017).</p> |
<h2>Experiments</h2> | <h2>Experiments</h2> | ||
Revision as of 14:29, 15 October 2020
Geraniol synthase from Catharanthus roseus
Usage
Catharanthus roseus produces monoterpene indole alkaloids (MIAs). Geraniol synthase catalyzes a key step in the generation of geraniol from geranyl diphosphate (GPP) in the monoterpenoid branch of the MIA pathway.
The sequence of the geraniol synthase from Catharanthus roseus (CrGES) has been taken from Genbank (JN882024.1), adapted for the usage in S. cerevisiae and then truncated to the 43 amino acid in order to increase its activity (Jiang et al., 2017).
Experiments
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
Related parts on regisrty
There is another part in registry BBa_K2753003, which corresponds to geraniol synthase. The two main differences are that: the sequence originates from Ocimum basilica and the codons are adapted for the usage in E.coli.
References
- GenBank ID: JN882024.1
- Jiang, G.-Z., Yao, M.-D., Wang, Y., Zhou, L., Song, T.-Q., Liu, H., Xiao, W.-H., & Yuan, Y.-J. (2017). Manipulation of GES and ERG20 for geraniol overproduction in Saccharomyces cerevisiae. Metabolic Engineering, 41, 57–66. https://doi.org/10.1016/j.ymben.2017.03.005
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]