Difference between revisions of "Part:BBa K3570009"
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<h2>Usage</h2>
 
<h2>Usage</h2>
 
HO upstream homology arm part shall be used together with HO downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570010 BBa_K3570010]) to target a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae'</i>s genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.
 
HO upstream homology arm part shall be used together with HO downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570010 BBa_K3570010]) to target a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae'</i>s genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.
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<p style="text-indent: 40px">
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This sequence was identified from a personal communication from Dr. Gilles Truan. </p>
  
 
<h2>Experiments</h2>
 
<h2>Experiments</h2>

Revision as of 14:02, 15 October 2020


HO upstream homologous sequence

Usage

HO upstream homology arm part shall be used together with HO downstream homology arm part (BBa_K3570010) to target a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.

This sequence was identified from a personal communication from Dr. Gilles Truan.

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References

  • [1]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]