Difference between revisions of "Part:BBa K3360003"

 
Line 3: Line 3:
 
<partinfo>BBa_K3360003 short</partinfo>
 
<partinfo>BBa_K3360003 short</partinfo>
  
−
ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTGGATCCATGAAACGTAGCATTAGCATCTTCATTACCTGCCTGCTGATCACCCTGCTGACCATGGGTGGCATGATGGCGAGCCCGGCGAGCGCGGCGGGTACCAAAACCCCGGTTGCGAAGAACGGCCAGCTGAGCATCAAGGGTACCCAACTGGTTAACCGTGATGGTAAAGCGGTGCAGCTGAAGGGTATTAGCAGCCACGGCCTGCAATGGTACGGTGAATATGTTAACAAAGACAGCCTGAAGTGGCTGCGTGACGATTGGGGTATCACCGTGTTTCGTGCGGCGATGTACACCGCGGATGGTGGCTATATTGATAACCCGAGCGTGAAGAACAAAGTTAAAGAGGCGGTGGAAGCGGCGAAAGAGCTGGGCATCTACGTGATCATTGACTGGCACATTCTGAACGATGGTAACCCGAACCAGAACAAAGAGAAGGCGAAAGAATTCTTTAAGGAGATGAGCAGCCTGTACGGCAACACCCCGAACGTTATCTATGAAATTGCGAACGAGCCGAACGGTGACGTGAACTGGAAACGTGATATCAAGCCGTATGCGGAGGAAGTGATCAGCGTTATTCGTAAAAACGACCCGGATAACATCATTATCGTTGGTACCGGCACCTGGAGCCAGGACGTGAACGATGCGGCGGACGATCAACTGAAGGATGCGAACGTTATGTACGCGCTGCACTTCTATGCGGGTACCCACGGCCAATTTCTGCGTGACAAAGCGAACTACGCGCTGAGCAAGGGTGCGCCGATCTTCGTTACCGAATGGGGTACCAGCGATGCGAGCGGTAACGGTGGCGTGTTTCTGGATCAGAGCCGTGAGTGGCTGAAATATCTGGACAGCAAGACCATTAGCTGGGTGAACTGGAACCTGAGCGATAAACAAGAAAGCAGCAGCGCGCTGAAGCCGGGTGCGAGCAAGACCGGTGGCTGGCGTCTGAGCGACCTGAGCGCGAGCGGCACCTTCGTTCGTGAGAACATCCTGGGTACCAAAGACAGCACCAAGGATATTCCGGAAACCCCGAGCAAGGATAAACCGACCCAGGAGAACGGTATCAGCGTGCAATACCGTGCGGGTGACGGCAGCATGAACAGCAACCAGATCCGTCCGCAGCTGCAAATTAAAAACAACGGCAACACCACCGTTGACCTGAAGGATGTGACCGCGCGTTACTGGTATAAGGCGAAAAACAAGGGTCAGAACGTTGACTGCGATTACGCGCAAATCGGTTGCGGCAACGTTACCCACAAATTCGTGACCCTGCACAAACCGAAGCAAGGCGCGGACACCTATCTGGAACTGGGTTTTAAGAACGGTACCCTGGCGCCGGGTGCGAGCACCGGCAACATTCAGCTGCGTCTGCACAACGACGATTGGAGCAACTACGCGCAAAGCGGTGATTACAGCTTCTTTAAGAGCAACACCTTCAAGACCACCAAGAAAATCACCCTGTACGACCAGGGCAAGCTGATTTGGGGTACCGAGCCGAACTAAGCGGCC
+
Encoding the endo-beta-1,4-glucanase of Bacillus subtilis, the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:46, 15 October 2020


IARI-SP-2

Encoding the endo-beta-1,4-glucanase of Bacillus subtilis, the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]