Difference between revisions of "Part:BBa K3394001"
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[[File:BBa_K3394001-CBD_image.png|200px|thumb|left|Figure 1: CBD - Cellulose-Binding Domains]] | [[File:BBa_K3394001-CBD_image.png|200px|thumb|left|Figure 1: CBD - Cellulose-Binding Domains]] | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:23, 15 October 2020
Coding Sequence of CBD (Cellulose Binding Domain)
Most cellulolytic complexes consist of catalytic domains (Cellulases) and Cellulose-Binding Domains (CBDs).
The binding domain improves the attachment of the enzyme to the substrate and facilitate the catalytic domain activity. Similar binding domain structures can be found in complexes degrading other insoluble carbohydrates such as chitin.
The CBD sequence we used was cloned into an Escherichia coli expression vector (PET-28) in the lab.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 37
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 37
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 37
Illegal AgeI site found at 12 - 1000COMPATIBLE WITH RFC[1000]