Difference between revisions of "Part:BBa K3585001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | In order to meet the typeIIS standard, we made a synonymous mutation of Asp50Asp and Leu137Leu to remove BsaI restriction site | + | In order to meet the typeIIS standard, we made a synonymous mutation of Asp50Asp and Leu137Leu to remove BsaI restriction site. |
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===Source=== | ===Source=== | ||
Latest revision as of 19:27, 14 October 2020
Biosynthetic gene cluster of butyrate synthesis pathway enzymes
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1175
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6207
Illegal BglII site found at 7343
Illegal BglII site found at 7484
Illegal XhoI site found at 1180 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to meet the typeIIS standard, we made a synonymous mutation of Asp50Asp and Leu137Leu to remove BsaI restriction site.
Source
It comes from the genomic sequence of Clostridium beijerinckii.