Difference between revisions of "Part:BBa K3521005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We mutated C to A to remove the SapI | + | We mutated C to A to remove the SapI restriction site to meet the type IIS standard. |
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===Source=== | ===Source=== | ||
Revision as of 17:58, 14 October 2020
pT7-FnCpf1-His tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1784
Illegal NheI site found at 3504 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 3565
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We mutated C to A to remove the SapI restriction site to meet the type IIS standard.
Source
The sequence of FnCpf1 comes from the genomic sequence of Francisella novicida.