Difference between revisions of "Part:BBa K3425033:Design"

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===Design Notes===
 
===Design Notes===
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Spacer provides optimal distance for E. coli when Type IIS iGEM Standard assembly is implemented.
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The spacer provides aligned spacing (the distance between RBS and ATG) of 10 nucleotides when Type IIS iGEM Standard assembly is used.
  
 
===Source===
 
===Source===

Revision as of 10:19, 14 October 2020


iGEM Type IIS pSB1K03-DY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacer provides aligned spacing (the distance between RBS and ATG) of 10 nucleotides when Type IIS iGEM Standard assembly is used.

Source

iGEM Registry of Standard Parts BBa_B0030

References

For aligned spacing refer:

Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745