Difference between revisions of "Part:BBa K3570010"
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<h2>Usage</h2> | <h2>Usage</h2> | ||
− | HO downstream homology arm part used together with HO upstream homology arm part ([https://parts.igem.org/Part:BBa_K3570009 BBa_K3570009]). When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene. | + | HO downstream homology arm part used together with HO upstream homology arm part ([https://parts.igem.org/Part:BBa_K3570009 BBa_K3570009]) to target a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae</i>'s genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene. |
<h2>Experiments</h2> | <h2>Experiments</h2> |
Revision as of 04:49, 14 October 2020
HO downstream homologous sequence
Usage
HO downstream homology arm part used together with HO upstream homology arm part (BBa_K3570009) to target a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.
Experiments
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
References
- [1]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]