Difference between revisions of "Part:BBa K3570006"
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<h2>Usage</h2> | <h2>Usage</h2> | ||
<p style="text-indent: 40px"> | <p style="text-indent: 40px"> | ||
| − | DPP1 upstream homology arm part shall be used together with DPP1 downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570007 BBa_K3570007]) to | + | DPP1 upstream homology arm part shall be used together with DPP1 downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570007 BBa_K3570007]) to target a functional yeast integration locus. When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae's</i> genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene. </p> |
<h2>Experiments</h2> | <h2>Experiments</h2> | ||
Revision as of 04:46, 14 October 2020
DPP1 upstream homologous sequence
Usage
DPP1 upstream homology arm part shall be used together with DPP1 downstream homology arm part (BBa_K3570007) to target a functional yeast integration locus. When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.
Experiments
References
S. cerevisiae genome, chromosome IV, DPP1 gene. GenBank: CP046084.1
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 50
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 50
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 50
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 50
- 1000COMPATIBLE WITH RFC[1000]