Difference between revisions of "Part:BBa K3570008"
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HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.</p> | HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.</p> | ||
<p style="text-indent: 40px"> | <p style="text-indent: 40px"> | ||
− | HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, | + | HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the prototroph character for histidine. Those cells should not have the functional HIS3 gene in its genome[1]. </p> |
<p style="text-indent: 40px"> | <p style="text-indent: 40px"> | ||
The sequence contains HIS3 specific promoter, HIS3 coding sequence, and HIS3 terminator.</p> | The sequence contains HIS3 specific promoter, HIS3 coding sequence, and HIS3 terminator.</p> |
Revision as of 17:40, 13 October 2020
HIS3 selection marker
Usage
HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.
HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the prototroph character for histidine. Those cells should not have the functional HIS3 gene in its genome[1].
The sequence contains HIS3 specific promoter, HIS3 coding sequence, and HIS3 terminator.
Experiments
References
- [1]- Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
- [2]- GenBank: U03439.1
- [3]- RS313 plasmid
- [4]- SGD:S000005728
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1005
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 896
Illegal BglII site found at 956 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]