Difference between revisions of "Part:BBa K3570015"
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<partinfo>BBa_K3570015 short</partinfo> | <partinfo>BBa_K3570015 short</partinfo> | ||
− | YPRCdelta15 downstream homologous arm used together with YPRCdelta15 upstream (BBa_K3570014). When YPRCdelta15 upstream put to 5' of the biobrick together with YPRCdelta15 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do | + | YPRCdelta15 downstream homologous arm part shall be used together with YPRCdelta15 upstream homology arm part ([https://parts.igem.org/Part:BBa_K3570014 BBa_K3570014]). When YPRCdelta15 upstream put to 5' of the biobrick together with YPRCdelta15 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within a region of type 1 long terminal repeat (Ty1 LTR) in chromosome XVI. |
+ | |||
+ | <h2>Experiments</h2> | ||
+ | Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality. | ||
+ | <h2>References</h2> | ||
+ | <i>S. cerevisiae</i> genome, chromosome XVI, Ty1 LTR region. GenBank: CP046096.1 | ||
+ | |||
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Revision as of 17:16, 13 October 2020
YPRCdelta15 downstream homologous sequnce
YPRCdelta15 downstream homologous arm part shall be used together with YPRCdelta15 upstream homology arm part (BBa_K3570014). When YPRCdelta15 upstream put to 5' of the biobrick together with YPRCdelta15 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within a region of type 1 long terminal repeat (Ty1 LTR) in chromosome XVI.
Experiments
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
References
S. cerevisiae genome, chromosome XVI, Ty1 LTR region. GenBank: CP046096.1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]