Difference between revisions of "Part:BBa K3570000"

Revision as of 15:45, 13 October 2020

GGPP production enhancement in S. cerevisiae

Introduction

The ultimate goal of this biobrick is to enhance the mevalonate pathway in S. Cerevisiae to increase the pool of geranylgeranyl pyrophosphate (GGPP). The surplus of GGPP can be used to make S. Cerevisiae produce provitamin A (𝛽-carotene), geraniol or limonene using BBa_K3570001, BBa_K3570002 or BBa_K3570003 biobricks respectively(figure 1).

Fig. 1: Metabolic pathway of 𝛽-carotene, limonene and geraniol from Acetyl-CoA in engineered yeast.

Design

According to Rabeharindranto et al. 2019, the enhancement of the mevalonate pathway can be achieved by overexpressing the gene HMG1 and CrtE. The construction as it is presented here differs from the publication in the choice of the promoter. The promoter TDH1 has been chosen instead of Gal1/10 because it was needed elsewhere in our project iGEMINI. We thus created the plasmids containing a truncated version of the HMG1 gene from S. Cerevisiae and the CrtE gene from X. Dendrorhous as on figure 2.

Fig. 2: HMG1-CrtE-pUC19. The integrative locus used is DPP. The selective locus used is HIS. The genes coding for the truncated version of HMG1 comes from S. cerevisiae. The genes coding for CrtE comes from X. dendrorhous. We use the bidirectional TDH3-TEF1 promoter and the terminators tCYC1 and tPGK.

Refernces

• [1]- Hery Rabeharindranto, Sara Castaño-Cerezo, Thomas Lautier, Luis F. Garcia-Alles, Christian Treitz, Andreas Tholey, Gilles Truan, 2019. Enzyme-fusion strategies for redirecting and improving carotenoid synthesis in S. cerevisiae. Metab Eng Commun. 2019 Jun; 8: e00086.

Sequence and Features