Difference between revisions of "Part:BBa K3504011"
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==Characterization==
 
==Characterization==
 
==Improvements==
 
==Improvements==
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We used multiple datasets which we filtered according to the more vicious traits of The tumor to know the most expressed genes in these samples ,and then according to logfc & t value by which we were able to minimize the data in them. Using 3 datasets we tried to find intersections between them using tools such as genevenn, in which by this we detected a list of 26 shared neoantigens to work on. Also this selection was done using literature mining techniques to find clinically used and tried neo-epitopes.
 
  
 
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Revision as of 08:05, 13 October 2020


Multi-Epitope TNBC Vaccine Version (1)

Part Description

A multi-epitope vaccine formed of highly expressed and specific TNBC neo-epitopes and specifically chosen according to egyptian population alleles which can work as a generalized vaccine and also personalized vaccine which would illicit an immune response specific to TNBC tumor cells

Usage

Characterization

Improvements

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 120
    Illegal NotI site found at 44
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 120
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 584
    Illegal BsaI.rc site found at 935
    Illegal SapI.rc site found at 853