Difference between revisions of "Part:BBa K3570013"
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The URA3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Orotidine-5'-phosphate (OMP) decarboxylase which catalyzes the sixth step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP). </p>
 
The URA3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Orotidine-5'-phosphate (OMP) decarboxylase which catalyzes the sixth step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP). </p>
 
<p style="text-indent: 40px">
 
<p style="text-indent: 40px">
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When inserted into an integrative or replicative plasmid, URA3 allows to counter-select the cells that acquired the wanted plasmid.</p>
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URA3 gene serves as a commonly used yeast selectable marker. When inserted into an integrative or replicative plasmid, URA3 allows to counter-select the cells that acquired prototroph character for uracil. Those cells should not have the functional HIS3 gene in its genome[1].</p>
  
 
<h2>Experiments</h2>
 
<h2>Experiments</h2>
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<h2>References:</h2>
 
<h2>References:</h2>
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Taken from FL38 plasmid (https://www.addgene.org/vector-database/2750/)
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*[1]- Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
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*[2]- [https://www.addgene.org/vector-database/2750/ FL38 plasmid]
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*[3]- [https://www.yeastgenome.org/locus/s000000747 SGD:S000000747]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:29, 13 October 2020


URA3 selection marker


Biology

The URA3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Orotidine-5'-phosphate (OMP) decarboxylase which catalyzes the sixth step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP).

URA3 gene serves as a commonly used yeast selectable marker. When inserted into an integrative or replicative plasmid, URA3 allows to counter-select the cells that acquired prototroph character for uracil. Those cells should not have the functional HIS3 gene in its genome[1].

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References:

  • [1]- Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
  • [2]- FL38 plasmid
  • [3]- SGD:S000000747

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
    Illegal BglII site found at 1374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]