Difference between revisions of "Part:BBa K3570018"
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<partinfo>BBa_K3570018 short</partinfo> | <partinfo>BBa_K3570018 short</partinfo> | ||
− | The MET17 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a bifunctional enzyme with O-acetylserine and O-acetylhomoserine sulfhydrylase activities involved in methionine and cysteine metabolism. It | + | <h2>Usage</h2> |
+ | <p style="text-indent: 40px"> | ||
+ | The MET17 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a bifunctional enzyme with O-acetylserine and O-acetylhomoserine sulfhydrylase activities involved in methionine and cysteine metabolism. It catalyzes the reaction between acetylated serine or homoserine with thiol to produce the corresponding amino acid and is localized to the cytoplasm and plasma membrane. </p> | ||
− | When inserted into an integrative or replicative plasmid, MET17 allows to counter-select the cells that acquired the prototroph character for methionine. | + | <p style="text-indent: 40px"> |
+ | When inserted into an integrative or replicative plasmid, MET17 allows to counter-select the cells that acquired the prototroph character for methionine.</p> | ||
− | References | + | <h2>Experiments</h2> |
+ | Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality. | ||
+ | |||
+ | <h2>References</h2> | ||
*[1]- [https://www.yeastgenome.org/locus/S000004294 SGD:S000004294] | *[1]- [https://www.yeastgenome.org/locus/S000004294 SGD:S000004294] | ||
Revision as of 07:16, 13 October 2020
MET17 selection marker
Usage
The MET17 gene, found in the Saccharomyces cerevisiae yeast, encodes a bifunctional enzyme with O-acetylserine and O-acetylhomoserine sulfhydrylase activities involved in methionine and cysteine metabolism. It catalyzes the reaction between acetylated serine or homoserine with thiol to produce the corresponding amino acid and is localized to the cytoplasm and plasma membrane.
When inserted into an integrative or replicative plasmid, MET17 allows to counter-select the cells that acquired the prototroph character for methionine.
Experiments
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
References
- [1]- SGD:S000004294
- [2]- Masselot, M., & de Robichon-Szulmajster, H. (1975). Methionine biosynthesis in Saccharomyces cerevisiae. Molecular and General Genetics MGG, 139(2), 121–132. https://doi.org/10.1007/bf00264692
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 649
Illegal XbaI site found at 378 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 649
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 649
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 649
Illegal XbaI site found at 378 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 649
Illegal XbaI site found at 378
Illegal NgoMIV site found at 268 - 1000COMPATIBLE WITH RFC[1000]