Difference between revisions of "Part:BBa K3570012"

Revision as of 05:22, 13 October 2020

Geraniol synthase from Catharanthus roseus

Usage

Catharanthus roseus produces monoterpene indole alkaloids (MIAs). Geraniol synthase catalyzes a key step in the generation of geraniol from geranyl diphosphate (GPP) in the monoterpenoid branch of the MIA pathway.

The sequence of the geraniol synthase from Catharanthus roseus (CrGES) has been taken from Genbank (JN882024.1) then truncated to the 43 amino acid in order to increase its activity (Jiang et al., 2017).

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References

GenBank ID: JN882024.1

Jiang, G.-Z., Yao, M.-D., Wang, Y., Zhou, L., Song, T.-Q., Liu, H., Xiao, W.-H., & Yuan, Y.-J. (2017). Manipulation of GES and ERG20 for geraniol overproduction in Saccharomyces cerevisiae. Metabolic Engineering, 41, 57–66. https://doi.org/10.1016/j.ymben.2017.03.005

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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+<h2>Usage</h2>
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 The sequence of the geraniol synthase from <i>Catharanthus roseus</i> (CrGES) has been taken from Genbank (JN882024.1) then truncated to the 43 amino acid in order to increase its activity (Jiang <i>et al</i>., 2017).</p>
-References:
+<h2>Experiments</h2>
+Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
+<h2>References</h2>
 * GenBank ID: JN882024.1