Difference between revisions of "Part:BBa K3570009"
| Line 9: | Line 9: | ||
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality. | Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality. | ||
| − | <h2> | + | <h2>References</h2> |
*[1]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1 | *[1]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1 | ||
Revision as of 05:21, 13 October 2020
HO upstream homologous sequence
Usage
HO upstream homology arm part shall be used together with HO downstream homology arm part (BBa_K3570010) to form a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.
Experiments
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
References
- [1]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]