Difference between revisions of "Part:BBa K3570008"
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<h2>Usage</h2> | <h2>Usage</h2> | ||
− | + | <p style="text-indent: 40px"> | |
+ | HIS3 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli. | ||
+ | HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, it selects and maintains a yeast vector in yeast host cells[1]. </p> | ||
+ | <p style="text-indent: 40px"> | ||
+ | The sequence contains HIS3 specific promoter, HIS3 coding sequence, and HIS3 terminator.</p> | ||
− | |||
<h2>Source</h2> | <h2>Source</h2> | ||
− | + | *[1]Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press. | |
− | *[1]GenBank: U03439.1 | + | *[2]GenBank: U03439.1 |
− | *[ | + | *[3]RS313 plasmid https://www.addgene.org/vector-database/3972/ |
− | *[ | + | *[4]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3 |
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Revision as of 18:52, 12 October 2020
HIS3 selection marker
Usage
HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli. HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, it selects and maintains a yeast vector in yeast host cells[1].
The sequence contains HIS3 specific promoter, HIS3 coding sequence, and HIS3 terminator.
Source
- [1]Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
- [2]GenBank: U03439.1
- [3]RS313 plasmid https://www.addgene.org/vector-database/3972/
- [4]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1005
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 896
Illegal BglII site found at 956 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]