Difference between revisions of "Part:BBa K2117000"

Revision as of 07:18, 12 October 2020

Constitutive TEF1 promoter native to Yarrowia lipolytica

Usage and Biology

The translation elongation factor-1α (TEF1) promoter is a strong constitutive promoter. Native for the oleaginous yeast Yarrowia lipolytica.

This promoter is often used for isolation of enzyme genes by expression cloning. However, the promoter is usually not recommended for heterologous production due to early expression of heterologous genes can be detrimental for cell growth.

Improvement by Evry_Paris-Saclay 2019

This part is functional as a promoter in the oleaginous yeast Yarrowia lipolytica, but it is not compatible with the Type IIS RFC[1000] standard and thus cannot be used in cloning experiments using the Golden Gate technique. As this is a powerful molecular biology technique that allows scarless assembly of a large number of DNA fragments and now it is fully supported by iGEM, we improved this part to make it compatible with the Type IIS RFC[1000] standard.

Thus, we have successfully built 4 versions of the pTef1 promoter: pTef1c (BBa_K2983050), pTef1d (BBa_K2983051), pTef1e (BBa_K2983052) and pTef1f (BBa_K2983053) which are able to drive the expression of a reporter gene in the oleaginous yeast Yarrowia lipolytica at an equivalent strength as this part already present in the iGEM Registry, pTef1a (BBa_K2117000).

Full details are available on the improved pTef1 promoter variants Part's Main Pages: BBa_K2983050, BBa_K2983051, BBa_K2983052 and BBa_K2983053.

Characterization by iGEM20_Calgary

Sequence and Features