Difference between revisions of "Part:BBa K3629010:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This is a long gene that might have to be split in two parts when ordered from a DNA synthesizing company.
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This is a long gene that might have to be split in two parts when ordered from a DNA synthesizing company. It was also codon optimized for expression and function in <i>Yarrowia lipolytica.</i>
  
  

Latest revision as of 05:32, 12 October 2020


Neocallimastix patriciarum BGS with 6X His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 2085
    Illegal BamHI site found at 417
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 928
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a long gene that might have to be split in two parts when ordered from a DNA synthesizing company. It was also codon optimized for expression and function in Yarrowia lipolytica.


Source

From genomic sequence of Neocallimastix patriciarum.

References