Difference between revisions of "Part:BBa K3629004:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
From the genomic sequence of <i> Y. lipolytica </i> strain CLIB122.
+
From the genomic sequence of wild-type <i> Y. lipolytica </i> strain CLIB122.
  
 
===References===
 
===References===

Revision as of 05:24, 12 October 2020


Yarrowia lipolytica XPR2 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 271
    Illegal EcoRI site found at 265
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 265
    Illegal SpeI site found at 272
    Illegal PstI site found at 286
    Illegal NotI site found at 7
    Illegal NotI site found at 279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 265
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 272
    Illegal EcoRI site found at 265
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 265
    Illegal XbaI site found at 16
    Illegal SpeI site found at 272
    Illegal PstI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Last 6nts contains an EcoRI restriction site.


Source

From the genomic sequence of wild-type Y. lipolytica strain CLIB122.

References