Difference between revisions of "Part:BBa K3629004"

 
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__NOTOC__
 
__NOTOC__
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{|width='80%'
 
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|-
 
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|width=35% valign='top'|
 
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[[Image:Terminator.png]]
 
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<partinfo>BBa_K3629004 parameters</partinfo>
 
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| width=50% valign='top' style='border: 1px solid black'|
 
 
<partinfo>BBa_K3629004 short</partinfo>
 
<partinfo>BBa_K3629004 short</partinfo>
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* Terminator sequence from the <i>Yarrowia lipolytica</i> alkaline extracellular protease XRP2 gene.
 
  
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|}
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TEF1 promoter from <i>Yarrowia lipolytica</i> and the first 42nts (14 codons) of the Lip2 signal peptide from <i>Y. lipolytica</i> [https://parts.igem.org/Part:BBa_K1592000 (BBa_K1592000)]
  
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'''Secondary Structure'''
 
  
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[[Image:Mfold-K3629004-1.png]]
 
  
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<hr>
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__TOC__
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'''Measurement'''
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===Usage and Biology===
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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===Design===
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===Sequence and Features===
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<partinfo>BBa_K3629004 SequenceAndFeatures</partinfo>
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===References===
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3629004 parameters</partinfo>
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<!-- -->

Revision as of 04:05, 12 October 2020


Yarrowia lipolytica XPR2 terminator

TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide from Y. lipolytica (BBa_K1592000)


Usage and Biology

Design

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 271
    Illegal EcoRI site found at 265
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 265
    Illegal SpeI site found at 272
    Illegal PstI site found at 286
    Illegal NotI site found at 7
    Illegal NotI site found at 279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 265
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 272
    Illegal EcoRI site found at 265
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 265
    Illegal XbaI site found at 16
    Illegal SpeI site found at 272
    Illegal PstI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]

References