Difference between revisions of "Part:BBa K3629019"
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2-isopropylmalate (LEU4) gene from <i>Yarrowia lipolytica </i>. | 2-isopropylmalate (LEU4) gene from <i>Yarrowia lipolytica </i>. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 03:53, 12 October 2020
Yarrowia lipolytica LEU4 gene
2-isopropylmalate (LEU4) gene from Yarrowia lipolytica .
Usage and Biology
The YALI0B07447g is the gene for Yarrowia lipolytica's 2-isopropylmalate synthase which is a LEU4 or
2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme specially on a strong promoter could be used to upregulate leucine synthesis and produce a leucine overproducing strain.
Design
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 728
Illegal BamHI site found at 293
Illegal XhoI site found at 428 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 799
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 544