Difference between revisions of "Part:BBa K3629019"

Revision as of 03:51, 12 October 2020

Yarrowia lipolytica LEU4 gene

2-isopropylmalate (LEU4) gene from Yarrowia lipolytica .

Usage and Biology

The YALI0B07447g is the gene for Yarrowia lipolytica's 2-isopropylmalate synthase which is a LEU4 or

2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme specially on a strong promoter could be used to upregulate leucine synthesis and produce a leucine overproducing strain.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 728
Illegal BamHI site found at 293
Illegal XhoI site found at 428
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 799
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 544

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 <partinfo>BBa_K3629019 short</partinfo>
--isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme specially on a strong promoter could be used to upregulate leucine synthesis and produce a leucine overproducing strain.
+-isopropylmalate (LEU4) gene from <i>Yarrowia lipolytica </i>.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The YALI0B07447g is the gene for Yarrowia lipolytica's 2-isopropylmalate synthase which is a LEU4 or
+-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme specially on a strong promoter could be used to upregulate leucine synthesis and produce a leucine overproducing strain.
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