Difference between revisions of "Part:BBa K3629003"

Revision as of 23:14, 11 October 2020

Yarrowia lipolytica EXP Promoter

The part contains wild-type TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide from Y. lipolytica (BBa_K1592000).

Upon digestion with BbsI and this part can be assembled into a destination vector (containing BBa_K3629012) with a coding sequence that contains the last 57nts (19 codons) of the Lip2 signal peptide at the 5' end by using Gibson Assembly. In our part collection, this part can be assembled with ________ into the BBa_K3629012 destination vector.

Usage and Biology

Figure 1. iGEM Calgary's genetic construct scheme. Each construct consists of a T7 inducible promoter, a strong RBS, one of six signal peptides, the water-soluble chlorophyll binding protein 6GIX with a 6X His tag, and a bidirectional terminator.

iGEM Calgary successfully created seven inducible genetic circuits for high-level production of 6GIX using various parts from our collection.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1025
Illegal SpeI site found at 602
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
Illegal NheI site found at 663
Illegal SpeI site found at 602
Illegal SpeI site found at 1026
Illegal PstI site found at 1040
Illegal NotI site found at 7
Illegal NotI site found at 1033
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1026
Illegal SpeI site found at 602
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 602
Illegal SpeI site found at 1026
Illegal PstI site found at 1040
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-[[Image:T--Calgary--6GIXConstructFinal.png|thumb|100px|right|center|Figure 1. iGEM Calgary's genetic construct scheme. Each construct consists of a T7 inducible promoter, a strong RBS, one of six signal peptides, the water-soluble chlorophyll binding protein 6GIX with a 6X His tag, and a bidirectional terminator.]]
+[[Image:T--Calgary--6GIXConstructFinal.png|thumb|500px|left|center|Figure 1. iGEM Calgary's genetic construct scheme. Each construct consists of a T7 inducible promoter, a strong RBS, one of six signal peptides, the water-soluble chlorophyll binding protein 6GIX with a 6X His tag, and a bidirectional terminator.]]
 iGEM Calgary successfully created seven inducible genetic circuits for high-level production of 6GIX using various parts from our [https://2019.igem.org/Team:Calgary/Part_Collection collection.]