Difference between revisions of "Part:BBa K3416000"

 
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=Introduction=
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==Biology==
  
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__NOTOC__
 
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<partinfo>BBa_K3416000 short</partinfo>
 
  
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Inducable promoter by AI-2, an interspecies signaling molecule.
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Quorum sensing (QS) is a communication system, which controls gene expression in response to population density, between bacteria. There are two QS systems: the first one, based on AI-1 or acyl-homoserine lactone (AHL), and the second – autoinducer 2<sup>1</sup>.
  
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AI-2 - interconverting molecules, which are derived from the same precursor and called the „universal“ bacterial signal<sup>1,2,3</sup> AI-2 controls the expression of LuxS regulated transporter, which is responsible for incorporation, phosphorylating, and processing of the AI-2 signal. The lsr transporter has genes, which expression is regulated by AI-2. LsrR is a repressor of the lsr operon. The AI-2, phosphorylated by lsrK, leads to derepression of lsr operon<sup>4</sup>.
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===Usage and Biology===
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===Description of ''lsrACDBFG''===
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<span class='h3bb'>Sequence and Features</span>
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''lsrACDBFGE'' is an operon that regulates the genes&apos; expression involved in AI-2 uptake and degradation. Autoinducer 2 (AI-2) is phosphorylated by LsrK, kinase, to phospho-AI-2. The phospho-AI-2 de-represses the ''lsrACDBFGE'' operon‘s repressor LsrR resulting in the induction of the genes9.  Hauk and her colleagues created promoters library from ''lsrACDBFG'' operon region. They have achieved two lsrACDBFG mutants, EP01rec and EP14rec, which has the same as WT function and successfully evolved<sup>5</sup>.
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<partinfo>BBa_K3416000 SequenceAndFeatures</partinfo>
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===References===
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#Stephens, K. & Bentley, W. E. Synthetic Biology for Manipulating Quorum Sensing in Microbial Consortia. ''Trends in Microbiology'' '''28''', 633–643 (2020).
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===Functional Parameters===
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#Sun, J., Daniel, R., Wagner-Döbler, I. & Zeng, A.-P. Is autoinducer-2 a universal signal for interspecies communication: a comparative genomic and phylogenetic analysis of the synthesis and signal transduction pathways. ''BMC Evol Biol 4'', '''36''' (2004).
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<partinfo>BBa_K3416000 parameters</partinfo>
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#Xavier, K. B. & Bassler, B. L. Interference with AI-2-mediated bacterial cell-cell communication. ''Nature'' '''437''', 750–753 (2005).
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#Xavier, K. B. et al. Phosphorylation and Processing of the Quorum-Sensing Molecule Autoinducer-2 in Enteric Bacteria. ''ACS Chem. Biol.'' '''2''', 128–136 (2007).
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#Hauk, P. ''et al.'' Insightful directed evolution of Escherichia coli quorum sensing promoter region of the lsrACDBFG operon: a tool for synthetic biology systems and protein expression. ''Nucleic Acids Res'' gkw981 (2016) doi:10.1093/nar/gkw981.

Revision as of 13:48, 11 October 2020

Introduction

Biology

Quorum sensing (QS) is a communication system, which controls gene expression in response to population density, between bacteria. There are two QS systems: the first one, based on AI-1 or acyl-homoserine lactone (AHL), and the second – autoinducer 21.

AI-2 - interconverting molecules, which are derived from the same precursor and called the „universal“ bacterial signal1,2,3 AI-2 controls the expression of LuxS regulated transporter, which is responsible for incorporation, phosphorylating, and processing of the AI-2 signal. The lsr transporter has genes, which expression is regulated by AI-2. LsrR is a repressor of the lsr operon. The AI-2, phosphorylated by lsrK, leads to derepression of lsr operon4.

Description of lsrACDBFG

lsrACDBFGE is an operon that regulates the genes' expression involved in AI-2 uptake and degradation. Autoinducer 2 (AI-2) is phosphorylated by LsrK, kinase, to phospho-AI-2. The phospho-AI-2 de-represses the lsrACDBFGE operon‘s repressor LsrR resulting in the induction of the genes9. Hauk and her colleagues created promoters library from lsrACDBFG operon region. They have achieved two lsrACDBFG mutants, EP01rec and EP14rec, which has the same as WT function and successfully evolved5.

References

  1. Stephens, K. & Bentley, W. E. Synthetic Biology for Manipulating Quorum Sensing in Microbial Consortia. Trends in Microbiology 28, 633–643 (2020).
  2. Sun, J., Daniel, R., Wagner-Döbler, I. & Zeng, A.-P. Is autoinducer-2 a universal signal for interspecies communication: a comparative genomic and phylogenetic analysis of the synthesis and signal transduction pathways. BMC Evol Biol 4, 36 (2004).
  3. Xavier, K. B. & Bassler, B. L. Interference with AI-2-mediated bacterial cell-cell communication. Nature 437, 750–753 (2005).
  4. Xavier, K. B. et al. Phosphorylation and Processing of the Quorum-Sensing Molecule Autoinducer-2 in Enteric Bacteria. ACS Chem. Biol. 2, 128–136 (2007).
  5. Hauk, P. et al. Insightful directed evolution of Escherichia coli quorum sensing promoter region of the lsrACDBFG operon: a tool for synthetic biology systems and protein expression. Nucleic Acids Res gkw981 (2016) doi:10.1093/nar/gkw981.