Difference between revisions of "Part:BBa K3408010"

 
Line 5: Line 5:
 
We used promoter P<sub>nar</sub> which was activated by FNR to trigger the expression of GFP. If there was no oxygen, then FNR could be transcribed and we could see green bacteria in our culture medium.
 
We used promoter P<sub>nar</sub> which was activated by FNR to trigger the expression of GFP. If there was no oxygen, then FNR could be transcribed and we could see green bacteria in our culture medium.
  
 +
 +
Plasmid profile
 +
https://2020.igem.org/wiki/images/3/37/T--NAU-CHINA--composite-parts2.1.png
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:59, 11 October 2020


Pnar-B0034-Trigger RNA-B0015-PCⅠ-Switch RNA-mazF-B0015

We used promoter Pnar which was activated by FNR to trigger the expression of GFP. If there was no oxygen, then FNR could be transcribed and we could see green bacteria in our culture medium.


Plasmid profile T--NAU-CHINA--composite-parts2.1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 187
    Illegal BglII site found at 409
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]