Difference between revisions of "Part:BBa K3408009"

Revision as of 08:58, 11 October 2020

P_liaG-trigger RNA-B0015-P_CⅠ-switch RNA-GFP-B0015

This composite part consists of promoter P_liaG and P_grac, lacI gene, CⅠ gene, GFP gene and some essential RBS and terminators. Promoter P_liaG starts transcription process and its transcription production is LacI, which is regulated by IPTG. When bacteria is exposed to medium which is rich in IPTG, bacteria can intake IPTG. IPTG is combined with LacI, then LacI cannot be bound to promoter Pgrac which is specially suppressed by LacI. Therefore, CⅠ repressor protein cannot generate as downstream cI gene cannot transcribe regularly. As a result, P_CⅠ recovers its activity and triggers the downstream transcription of GFP. By checking the expression of GFP, we could verify the feasibility of IPTG induction system, thus guaranteeing the successful culture of our engineered Bacillus subtilis.

Plasmid profile T--NAU-CHINA--composite-parts6.1.png Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 239
Illegal BglII site found at 461
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1190

@@ Line 6: / Line 6: @@
 As a result, P<sub>C&#8544;</sub> recovers its activity and triggers the downstream transcription of GFP. By checking the expression of GFP, we could verify the feasibility of IPTG induction system, thus guaranteeing the successful culture of our engineered <i>Bacillus subtilis</i>.
+Plasmid profile
+https://2020.igem.org/wiki/images/5/58/T--NAU-CHINA--composite-parts6.1.png
 <!-- Add more about the biology of this part here
 ===Usage and Biology===