Difference between revisions of "Part:BBa K3402011"

Latest revision as of 06:08, 11 October 2020

UGTB

UGTB, the gene of glucosyltransferase in Starmerella bombicola, is responsible for the second glycosylation step, verified by constructing UGTB gene deletion strain and measuring extracellular enzyme solution.

Usage and Biology

There are two independent UDP-glucosyltransferases existing during the synthesis of sophorolipids, UGTA and UGTB. UGTA is mainly responsible for the first step of glycosylation, and the knockout of this gene has no effect on the second step of glycosylation, but no sophorolipid is detected in the product. When UGTB was knocked out, sophorolipid was not detected in the product, but glucolipid could be detected. So UGTB is the key gene in the synthesis of sophorolipid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 834
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 256
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 415
Illegal BsaI.rc site found at 370

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3402011 short</partinfo>
-UGTB, the gene of glucosyltransferase in S. bombicola.
+<i>UGTB</i>, the gene of glucosyltransferase in <i>Starmerella bombicola</i>, is responsible for the second glycosylation step, verified by constructing <i>UGTB</i> gene deletion strain and measuring extracellular enzyme solution.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+There are two independent UDP-glucosyltransferases existing during the synthesis of sophorolipids, <i>UGTA</i> and <i>UGTB</i>. <i>UGTA</i> is mainly responsible for the first step of glycosylation, and the knockout of this gene has no effect on the second step of glycosylation, but no sophorolipid is detected in the product. When <i>UGTB</i> was knocked out, sophorolipid was not detected in the product, but glucolipid could be detected. So <i>UGTB</i> is the key gene in the synthesis of sophorolipid.
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