Difference between revisions of "Part:BBa K3402000"
| Line 8: | Line 8: | ||
We link different promoters to yeGFP to express green fluorescent protein, so we will know the strength of promoters by testing different fluorescence intensity of different promoters | We link different promoters to yeGFP to express green fluorescent protein, so we will know the strength of promoters by testing different fluorescence intensity of different promoters | ||
| − | [[Image: | + | [[Image:|400px|thumb|center|Fluorescence intensity of different promoters]] |
<!-- --> | <!-- --> | ||
Revision as of 02:38, 11 October 2020
yeGFP
The yeGFP can express green fluorescence protein, which can be used as a report gene to characterize the strength of promoters.
Usage and Biology
We link different promoters to yeGFP to express green fluorescent protein, so we will know the strength of promoters by testing different fluorescence intensity of different promoters
[[Image:|400px|thumb|center|Fluorescence intensity of different promoters]]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 13