Difference between revisions of "Part:BBa K3380501"
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The Edinburgh iGEM team 2020 designed a construct comprising a fluorescent RNA aptamer (Broccoli BBa_K3380153) flanked by a tRNA scaffold (F30 BBa_K3380101 and BBa_K3380102) under a strong class III T7 RNA polymerase promoter (BBa_z0251). It was designed to test the fluorescence intensity of the iSpinach aptamer flanked by F30 tRNA scaffold when binding to the DFHBI-1T fluorophore. The construct is capable of exhibiting fluorescence being a transcription only construct. The T7 RNA polymerase is capable of synthesizing transcripts via run-off transcription in the absence of a terminator. Figure 1 illustrates the detailed design of the construct. | The Edinburgh iGEM team 2020 designed a construct comprising a fluorescent RNA aptamer (Broccoli BBa_K3380153) flanked by a tRNA scaffold (F30 BBa_K3380101 and BBa_K3380102) under a strong class III T7 RNA polymerase promoter (BBa_z0251). It was designed to test the fluorescence intensity of the iSpinach aptamer flanked by F30 tRNA scaffold when binding to the DFHBI-1T fluorophore. The construct is capable of exhibiting fluorescence being a transcription only construct. The T7 RNA polymerase is capable of synthesizing transcripts via run-off transcription in the absence of a terminator. Figure 1 illustrates the detailed design of the construct. | ||
| + | [File:BBa K3380501.png] | ||
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Revision as of 09:50, 10 October 2020
Broccoli fluorescent RNA aptamer construct under T7 RNA polymerase promoter (BBa_z0251)
The Edinburgh iGEM team 2020 designed a construct comprising a fluorescent RNA aptamer (Broccoli BBa_K3380153) flanked by a tRNA scaffold (F30 BBa_K3380101 and BBa_K3380102) under a strong class III T7 RNA polymerase promoter (BBa_z0251). It was designed to test the fluorescence intensity of the iSpinach aptamer flanked by F30 tRNA scaffold when binding to the DFHBI-1T fluorophore. The construct is capable of exhibiting fluorescence being a transcription only construct. The T7 RNA polymerase is capable of synthesizing transcripts via run-off transcription in the absence of a terminator. Figure 1 illustrates the detailed design of the construct. [File:BBa K3380501.png]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 25