Difference between revisions of "Part:BBa K3380101:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We ordered the synthesized sequence. Originally ordered sequence: 5’ TCCCttgccatgtgtatgtggg 3’ with TCCC being a linker, to facilitate further manipulations (swapping of the part or attaching to different promoters). | + | We ordered the synthesized sequence. Originally ordered sequence: forward (5’ TCCCttgccatgtgtatgtggg 3’) and (5’AGTAcccacatacacatggcaa 3’) with TCCC and AGTA respectively being a linker, to facilitate further manipulations (swapping of the part or attaching to different promoters). |
| − | + | The forward and reverse sequences were phosphorylated, annealed and ligated to other parts using T4 DNA ligase. | |
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 08:58, 10 October 2020
F30 Upstream RNA aptamer scaffold
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We ordered the synthesized sequence. Originally ordered sequence: forward (5’ TCCCttgccatgtgtatgtggg 3’) and (5’AGTAcccacatacacatggcaa 3’) with TCCC and AGTA respectively being a linker, to facilitate further manipulations (swapping of the part or attaching to different promoters). The forward and reverse sequences were phosphorylated, annealed and ligated to other parts using T4 DNA ligase.
Source
Reengineered from a natural three-way junction structure.
References
Filonov, G.S., Kam, C.W., Song, W. and Jaffrey, S.R., 2015. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chemistry & biology, 22(5), pp.649-660.