Difference between revisions of "Part:BBa K3380101:Design"
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===Design Notes===
 
===Design Notes===
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Commonly used RNA scaffolds exhibit unexpected instability and cleavage in bacterial and mammalian cells, thus the negative points must be controlled. The reengineered F30 showed it can enhance the brightness of mammalian cells expressing cassette-tagged RNAs.
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We ordered the synthesized sequence. Originally ordered sequence: 5’ TCCCttgccatgtgtatgtggg 3’ with TCCC being a linker, to facilitate further manipulations (swapping of the part or attaching to different promoters).  
  
  

Revision as of 08:54, 10 October 2020


F30 Upstream RNA aptamer scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ordered the synthesized sequence. Originally ordered sequence: 5’ TCCCttgccatgtgtatgtggg 3’ with TCCC being a linker, to facilitate further manipulations (swapping of the part or attaching to different promoters).


Source

Reengineered from a natural three-way junction structure.


References

Filonov, G.S., Kam, C.W., Song, W. and Jaffrey, S.R., 2015. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chemistry & biology, 22(5), pp.649-660.