Difference between revisions of "Part:BBa K3380101"

 
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<partinfo>BBa_K3380101 short</partinfo>
 
<partinfo>BBa_K3380101 short</partinfo>
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Short RNA sequence that is transcribed into a tRNA scaffold, it can help improve the fluorescence of RNA aptamers such as iSpinach ( [https://parts.igem.org/Part:BBa_K3380150] BBa_K3380150)
  
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RNA aptamers can be expressed in cells to influence and image cellular processes. Aptamer folding is maintained by inserting the aptamers into highly structured RNA scaffolds. RNA scaffolds, the efficiency of F30 has been proved for Spinach-tagged RNAs in cells.
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RNA aptamers can be expressed in cells to influence and image cellular processes. Aptamer folding is maintained by inserting the aptamers into highly structured tRNA scaffolds. The F30 tRNA scaffold, besides facilitating the fluorescent RNA aptamer folding, helps protect it from being degraded by the RNAses.  
  
 
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Revision as of 08:13, 10 October 2020


F30 Upstream RNA aptamer scaffold Short RNA sequence that is transcribed into a tRNA scaffold, it can help improve the fluorescence of RNA aptamers such as iSpinach ( [1] BBa_K3380150)

RNA aptamers can be expressed in cells to influence and image cellular processes. Aptamer folding is maintained by inserting the aptamers into highly structured tRNA scaffolds. The F30 tRNA scaffold, besides facilitating the fluorescent RNA aptamer folding, helps protect it from being degraded by the RNAses.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]