Difference between revisions of "Part:BBa K3504001"
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==Improvements== | ==Improvements== | ||
Using information in literature we were able to increase the replicon cloning and functional ability by adding Q739L Mutation to NSP2 | Using information in literature we were able to increase the replicon cloning and functional ability by adding Q739L Mutation to NSP2 | ||
| − | + | [[Image:Q739L_Mutation.png|thumb|right|Figure 1. Q739L Mutation in Nsp2.]] | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 18:23, 9 October 2020
nSp2 Protease-Eastern equine encephalitis virus
Part Description
Nsp2 is one of four non structural proteins that together forms the main complex responsible for the synthesis positive-sense viral RNAs, results in the synthesis of both the genomic and subgenomic RNAs, of which the subgenomic RNA is produced in excess of the viral genome. Which allows the virus to self-replicate into millions of copies of the virus.
Usage
Characterization
Improvements
Using information in literature we were able to increase the replicon cloning and functional ability by adding Q739L Mutation to NSP2
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 487
Illegal SpeI site found at 435 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 487
Illegal SpeI site found at 435 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 487
Illegal BglII site found at 609
Illegal BamHI site found at 471
Illegal BamHI site found at 834 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 487
Illegal SpeI site found at 435 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 487
Illegal SpeI site found at 435
Illegal NgoMIV site found at 1276 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 739
Illegal BsaI.rc site found at 1386