Difference between revisions of "Part:BBa K3505003:Design"

 
 
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===References===
 
===References===
 +
*Lewis M. The lac repressor. <em>C R Biol.</em> 2005 Jun;328(6):521-48. doi: 10.1016/j.crvi.2005.04.004. PMID: 15950160.
 +
*Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional      regulators. <em>Nature</em> 403, 335-338 (2000).

Latest revision as of 15:17, 9 October 2020


LacI-Lac repressor GB compatible with B3-B5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning.


Source

E.Coli , sequence found through Addgene https://www.addgene.org/82021/

References

  • Lewis M. The lac repressor. C R Biol. 2005 Jun;328(6):521-48. doi: 10.1016/j.crvi.2005.04.004. PMID: 15950160.
  • Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000).