Difference between revisions of "Part:BBa K3407001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa (Figure 2). The Cry7Ca1 toxin (activated Cry7Ca1 protein) crystal structure has been published and can be found in the Protein Data Bank under the accession number 5ZI1 | + | The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa (Figure 2). The Cry7Ca1 toxin (activated Cry7Ca1 protein) crystal structure has been published and can be found in the Protein Data Bank under the accession number 5ZI1 <html><a href="#1">[1]</a></html. |
The Cry7Ca1 toxin gene was codon-optimized for Escherichia coli using the GenSmartTM Codon Optimization Tool (https://www.genscript.com/tools/gensmart-codon-optimization). The forbidden restriction enzymes sites are the following: | The Cry7Ca1 toxin gene was codon-optimized for Escherichia coli using the GenSmartTM Codon Optimization Tool (https://www.genscript.com/tools/gensmart-codon-optimization). The forbidden restriction enzymes sites are the following: | ||
Revision as of 11:44, 9 October 2020
Cry7Ca1 toxin from Bacillus thuringiensis strain BTH-13
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 634
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1334
Illegal XhoI site found at 313 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa (Figure 2). The Cry7Ca1 toxin (activated Cry7Ca1 protein) crystal structure has been published and can be found in the Protein Data Bank under the accession number 5ZI1 [1]