Difference between revisions of "Part:BBa K3380153"
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===Characterization=== | ===Characterization=== | ||
| + | The Broccoli aptamer was designed and described by [https://pubs.acs.org/doi/abs/10.1021/ja508478x Filonov ''et al.'' (2014)]. | ||
The Broccoli secondary structure folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below. | The Broccoli secondary structure folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below. | ||
Revision as of 09:08, 9 October 2020
Broccoli fluorescent RNA aptamer
Broccoli is a fluorescent RNA aptamer comprising a short ribonucleotide sequence that exhibits fluorescence upon binding to a specific fluorophore.
Characterization
The Broccoli aptamer was designed and described by Filonov et al. (2014). The Broccoli secondary structure folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]