Difference between revisions of "Part:BBa K3380500"
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<partinfo>BBa_K3380500 short</partinfo> | <partinfo>BBa_K3380500 short</partinfo> | ||
| − | + | The Edinburgh iGEM team 2020 designed a construct comprising a fluorescent RNA aptamer (iSpinach BBa_K3380150) flanked by a tRNA scaffold (F30 BBa_K3380101 and BBa_K3380102) under a strong class III T7 RNA polymerase promoter (BBa_z0251). It was designed to test the fluorescence intensity of the iSpinach aptamer flanked by F30 tRNA scaffold when binding to the DFHBI fluorophore. The construct is capable of exhibiting fluorescence being a transcription only construct. Simultaneously we tested the efficiency of transcription in the absence of a terminator. The T7 RNA polymerase is capable of synthesizing transcripts via run-off transcription in the absence of a terminator. | |
Figure 1 illustrates the detailed design of the construct. | Figure 1 illustrates the detailed design of the construct. | ||
[[File:Part BBa K3380500.png|700px|]] | [[File:Part BBa K3380500.png|700px|]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The construct functioning solely on transcription and having a small length of only 195 nucleotides has multiple advantages over the conventional fluorescent proteins. The time required for the fluorescence formation is much shorter than compared to the time needed for the synthesis of the fluorescent proteins. Moreover, there is less burden exhibited on the cell when expressing the transcription only construct. | ||
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| + | The construct can also be used in cell-free systems. | ||
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Revision as of 16:05, 8 October 2020
iSpinach fluorescent RNA aptamer construct under T7 RNA polymerase promoter (BBa_z0251)
The Edinburgh iGEM team 2020 designed a construct comprising a fluorescent RNA aptamer (iSpinach BBa_K3380150) flanked by a tRNA scaffold (F30 BBa_K3380101 and BBa_K3380102) under a strong class III T7 RNA polymerase promoter (BBa_z0251). It was designed to test the fluorescence intensity of the iSpinach aptamer flanked by F30 tRNA scaffold when binding to the DFHBI fluorophore. The construct is capable of exhibiting fluorescence being a transcription only construct. Simultaneously we tested the efficiency of transcription in the absence of a terminator. The T7 RNA polymerase is capable of synthesizing transcripts via run-off transcription in the absence of a terminator. Figure 1 illustrates the detailed design of the construct.
Usage and Biology
The construct functioning solely on transcription and having a small length of only 195 nucleotides has multiple advantages over the conventional fluorescent proteins. The time required for the fluorescence formation is much shorter than compared to the time needed for the synthesis of the fluorescent proteins. Moreover, there is less burden exhibited on the cell when expressing the transcription only construct.
The construct can also be used in cell-free systems.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 25