Difference between revisions of "Part:BBa K3380150:Design"

(→‎Design Notes)
(→‎Source)
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
iSPinach is an artificial RNA aptamer that was obtained using the [https://doi.org/10.1016/j.bioeng.2007.06.001/ SELEX]approach. It is based on the Spinach fluorescent RNA aptamer.
+
iSPinach is an artificial RNA aptamer that was obtained using the [https://www.sciencedirect.com/science/article/abs/pii/S1389034407000664?via%3Dihub/ SELEX]approach. It is based on the Spinach fluorescent RNA aptamer.
  
 
The iSpinach fluorescent RNA aptamer was synthesized
 
The iSpinach fluorescent RNA aptamer was synthesized

Revision as of 11:54, 8 October 2020


iSpinach fluorescent RNA aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The iSpinach RNA aptamer was flanked by the F30 tRNA scaffold BBa_K3380101and BBa_K3380102for increased fluorescence and stability.

Source

iSPinach is an artificial RNA aptamer that was obtained using the SELEXapproach. It is based on the Spinach fluorescent RNA aptamer.

The iSpinach fluorescent RNA aptamer was synthesized

References

Autour, A., Westhof, E. and Ryckelynck, M., 2016. iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications. Nucleic acids research, 44(6), pp.2491-2500.