Difference between revisions of "Part:BBa K3398001"

 
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===Usage and Biology===
 
===Usage and Biology===
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T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
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Revision as of 07:39, 8 October 2020


Enhanced T7 promoter 2

This part is a T7 promoter derived from BBa_I712074 with some point mutation according to previous research [1], and as a higher expression efficiency relative to BBa_I712074 by the fluorescence intensity when expressing EGFP. To express BioBricks under the control of this part, a T7 polymerase gene is needed in the chassis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]