Difference between revisions of "Part:BBa K3349005:Design"

Latest revision as of 15:13, 7 October 2020

Pectin Lyase PelC-GFP

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 829
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 215

Design Notes

This part was codon optimized for use in E. coli. A C-terminal His tag was added to the GFP reporter for purification.

Source

This part was originally taken from the uniprot database. The NCBI sequence ID is WP_123062310.1. The GFP sequence was also taken from uniprot (P42212).

References

1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.

Revision as of 16:07, 6 October 2020 (view source) Kristiturton (Talk \| contribs) (→‎References) ← Older edit		Latest revision as of 15:13, 7 October 2020 (view source) Kristiturton (Talk \| contribs) (→‎References)
Line 16:		Line 16:

	===References===		===References===
−	1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.	+	1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of <i>Paenibacillus amylolyticus</i> 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.