Difference between revisions of "Part:BBa K3349003"

Latest revision as of 15:08, 7 October 2020

Pectin Lyase Pnl-GFP

This Pectin lyase is originally from Paenabacillus amylolyticus. Pnl cleaves at methylated sites of pectin or homogalacturonan substrates at high affinity [1]. Used in conjunction with Pectin lyases PelB and PelC, pectin can be effectively degraded [1]. Keggi and Doran-Peterson found this pectinase had a Km of 0.13 mg/ml and a Vmax of 144.6+/- 1.894 iU/mg when a 80% methylated substrate was used. Optimal reaction temperature was found to be 55°C and a pH of 9-10 [1]. For more information, please visit our website https://2020.igem.org/Team:Lethbridge_HS or more analysis of the pnl gene see part BBa_K3349000.

A GFP reporter has been fused via a GC linker to the C terminal end of the protein. As well a C-terminal his tag was also added.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3349003 short</partinfo>
-This Pectin lyase is originally from <i> Paenabacillus amylolyticus </i>. Pnl cleaves at methylated sites of pectin or homogalacturonan substrates at high affinity [1]. Used in conjuction with Pectin lyases PelB and PelC, pectin can be effectively cleaved [1]. Keggi and Doran-Peterson found this pectinase had a Km of 0.13 mg/ml and a Vmax of 144.6+/- 1.894 iU/mg when a 80% methylated substrate was used. Optimal reaction temperature was found to be 55°C and between a pH of 9-10 [1]. For more information, please visit our website https://2020.igem.org/Team:Lethbridge_HS or more analysis of the pnl gene see part BBa_K3349000.
+This Pectin lyase is originally from <i> Paenabacillus amylolyticus</i>. Pnl cleaves at methylated sites of pectin or homogalacturonan substrates at high affinity [1]. Used in conjunction with Pectin lyases PelB and PelC, pectin can be effectively degraded [1]. Keggi and Doran-Peterson found this pectinase had a Km of 0.13 mg/ml and a Vmax of 144.6+/- 1.894 iU/mg when a 80% methylated substrate was used. Optimal reaction temperature was found to be 55°C and a pH of 9-10 [1]. For more information, please visit our website https://2020.igem.org/Team:Lethbridge_HS or more analysis of the pnl gene see part BBa_K3349000.
 A GFP reporter has been fused via a GC linker to the C terminal end of the protein. As well a C-terminal his tag was also added.