Difference between revisions of "Part:BBa I757007"

Revision as of 13:27, 7 October 2020

phyA1-406_N_part

[1] contributed to the characterisation of this part by adding a new documentation learned form literature on how to use Fhy1 and PhyA as a transcriptoinal optogenetic effectors.
(--antonmykhailiuk 15:26, 07 October 2020 (UTC+2))

PhyA is an interaction partner of Fhy1 (parts BBa_I757002, I757004)
Purpose: design of a light switchable interaction
this part codes for Amino acids 1-406, encompassing the potential Fhy1-interaction domain
N-terminus of this protein should not be fused for fusions to avoid disturbance of folding and potential Fhy1 interaction ("N-part")
SwissProt: P14712
NgoMIV / AgeI protein fusion part
iGEM Team Freiburg 2007
synthetic DNA by GeneArt optimized for E.coli
Part in pGA4 vector (AmpR, ColEI ori)

Contribution from other teams

Characterisation

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1219
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_I757007 short</partinfo>
+[https://2020.igem.org/Team:Toulouse_INSA-UPS] contributed to the characterisation of this part by adding a new documentation learned form literature on how to use Fhy1 and PhyA as a transcriptoinal optogenetic effectors. <br>
+(<small>--[[User:antonmykhailiuk|antonmykhailiuk]] 15:26, 07 October 2020 (UTC+2)</small>)
 * PhyA is an interaction partner of Fhy1 (parts BBa_I757002, I757004)
@@ Line 11: / Line 15: @@
 * synthetic DNA by GeneArt optimized for E.coli
 * Part in pGA4 vector (AmpR, ColEI ori)
+<h2>Contribution from other teams</h2>
+===Characterisation===
 <!-- Add more about the biology of this part here