Difference between revisions of "Part:BBa K3349005"

Revision as of 16:06, 6 October 2020

Pectin Lyase PelC-GFP

The PelC Pectin Lyase is originally from Paenabacillus amylolyticus . This particular pectin lyase is more specific to methylated pectin or homogalacturonan substrates rather than polygalacturonic acid [1]. This protein also works with both PelB(BBa_K3349001) and Pnl(BBa_K3349001) in this organism to effectively break down pectin. PelC has an optimal activity at 55°C and a pH of 10. The Km in in terms of 80% methylated substrates is 0.41 +/-0.06mg/ml. Calcium is a cofactor for this protein and is therefore necessary within the buffer conditions [1].

A GFP reporter was added onto the C-terminus. As well a C-terminal his tag was added to the GFp for nickel affinity purification.

For more information on our design and use please see our webpage https://2020.igem.org/Team:Lethbridge_HS or visit the PelC gene part page BBa_K3349002.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 829
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 215

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3349005 short</partinfo>
-The PelC Pectin Lyase is originally from <i> Paenabacillus amylolyticus</i>. A GFP reporter was added onto the C-terminus.
+The PelC Pectin Lyase is originally from Paenabacillus amylolyticus . This particular pectin lyase is more specific to methylated pectin or homogalacturonan substrates rather than polygalacturonic acid [1]. This protein also works with both PelB(BBa_K3349001) and Pnl(BBa_K3349001) in this organism to effectively break down pectin. PelC has an optimal activity at 55°C and a pH of 10. The Km in in terms of 80% methylated substrates is 0.41 +/-0.06mg/ml. Calcium is a cofactor for this protein and is therefore necessary within the buffer conditions [1].
+A GFP reporter was added onto the C-terminus. As well a C-terminal his tag was added to the GFp for nickel affinity purification.
+For more information on our design and use please see our webpage https://2020.igem.org/Team:Lethbridge_HS or visit the PelC gene part page BBa_K3349002.
 <!-- Add more about the biology of this part here