Difference between revisions of "Part:BBa K3588001"
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k<sub>cat</sub> is 9.4 sec<sup>-1</sup> | k<sub>cat</sub> is 9.4 sec<sup>-1</sup> | ||
K<sub>M</sub>=1.6 µM<ref>http://dx.doi.org/10.1021/bi026131p</ref> | K<sub>M</sub>=1.6 µM<ref>http://dx.doi.org/10.1021/bi026131p</ref> | ||
| + | |||
| + | <h5>Other Details</h5> | ||
| + | Half Life (at 37°C = ~6 mins) <ref>https://pubs.acs.org/doi/10.1021/cb500257s</ref> | ||
[[File:CocE Caracterisation.png|none|400px|thumb| ''pH dependence on cocE kinetics and stability.''<ref>scihub.wikicn.top/10.1021/bi026131p</ref>]] | [[File:CocE Caracterisation.png|none|400px|thumb| ''pH dependence on cocE kinetics and stability.''<ref>scihub.wikicn.top/10.1021/bi026131p</ref>]] | ||
Revision as of 15:38, 6 October 2020
CocE, Rhodococcus sp. (strain MB1 Bresler)
This part is the CDS of the enzyme Cocaine Esterase (CocE) from Rhodococcus sp. (strain MB1 Bresler). This enzyme hydrolyses cocaine faster than other known cocaine esterase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1188
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Function
The enzyme hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants. Also efficiently hydrolyzes cocaethylene, a more potent cocaine metabolite that has been observed in patients who concurrently abuse cocaine and alcohol. Is able to prevent cocaine-induced convulsions and lethality in rat.
Properties
Cocaine Substrate Kinetics
kcat is 7.8 sec-1 KM=0.64 µM[2]
Cocaethylene Substrate Kinetics
kcat is 9.4 sec-1 KM=1.6 µM[3]
Other Details
Half Life (at 37°C = ~6 mins) [4]
